AmaZulu FC will travel to the Old Peter Mokaba Stadium where they are scheduled to face Polokwane City on Saturday.
Kick-off is at 15h00 CAT.
Usuthu lost 3-0 against Bidvest Wits at home in their first game of the season last weekend.
Cavin Johnson’s side will be looking for an immediate response in this match in the Limpopo province.
Meanwhile, Polokwane opened their campaign on a winning note as they defeated Limpopo rivals Black Leopards 1-0 away last weekend.
Sibusiso Mbonani scored the only goal of the game for Zlatko Krmpotić’s side.
In head-to-head stats, Polokwane and AmaZulu have met in eight league matches since 2013/14.
Both Usuthu and Rise and Shine have claimed two wins apiece, while four matches have been drawn.
The Limpopo side has recorded one win and three draws from four home matches in the rivalry.
Last season, the teams drawing 0-0 in Durban in November 2018, before Polokwane won the return game 3-2 at the Old Peter Mokaba Stadium in January 2019.